In Vitro Antimicrobial Activity of Vanilla Mint Listerine Antiseptic Mouthrinse
S. Andreana1, D. Nittel-Ricci1, M.M. Wu1, D.S. Harper1, K.A. Baxter2, 1- Johnson & Johnson Consumer and Personal Products Worldwide, Morris Plains, NJ, USA; 2 - Hill Top Research, Miamiville, OH, USA. Poster # 2205 presented at IADR/AADR 2007 (New Orleans, LA, March 2007).
OBJECTIVES: To evaluate the in vitro antiseptic activity of Vanilla Mint Flavor Listerine® Antiseptic (VMLA) Mouthrinse.
METHODS: In vitro antimicrobial activity of less intense VMLA mouthrinse was compared to Cool Mint® Listerine® Mouthrinse (CMLA; positive control) and sterile water (negative control) in an in vitro kill kinetic critical time assay, in accordance with the relevant ADA Guideline. The products were tested against a panel of twenty representative oral and opportunistically infectious microorganisms, including species of Actinomyces, Campylobacter, Candida, Capnocytophaga, Elkenella, Fusobacterium, Actinobacillus, Lactobacillus, Leptotrichia, Porphyromonas, Prevotella, Pseudomonas, Staphylococcus, and Streptococcus. Each organism was grown under appropriate conditions, and subsequently exposed to the mouthrinses at a ratio of 1:9 v/v for 0.5, 1.0, and 2.0 minutes. At each time point an aliquot of 100 μ was placed in a neutralizing nutrient broth to inactivate the antimicrobial activity of the test solution and permit surviving organisms to grow. Turbidity was used as the end point. The shortest exposure time that resulted in no turbidity was considered the critical kill time. In separate tests, fetal bovine serum (FBS) was added to simulate biological fluids.
RESULTS: For all twenty microorganisms, VM LA and CMLA demonstrated a critical kill time of <0.5 minutes, both in the presence and absence of serum. Sterile water did not show any antimicrobial activity.
CONCLUSIONS: VMLA and CMLA mouthrinses exhibit antimicrobial activity versus representative oral microorganisms.
